Yeast Two-hybrid System
The Matchmaker Gold Yeast Two-Hybrid System is the most advanced and highest-performing version of our highly popular Matchmaker product line for investigating protein-protein interactions. This is due to a combination of a new yeast strain (Y2H Gold), stringent reporters, easy-to-use libraries, high-level expression vectors, and SMART-based library construction kits.
- Traditional Matchmaker Libraries
Supplied in E. coli, and therefore must be amplified and transformed into yeast before use
- Matchmaker Gold Yeast Two-Hybrid System
Most advanced and highest performing version of our Matchmaker product line for investigating protein-protein interactions
- Make Your Own Mate & Plate Library System
Provides the necessary components and a simple, highly efficient method to make your own Mate & Plate Library
- pGADT7 AD Vector
Designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein
- pGBKT7 DNA-BD Vector
Designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest
- Y187 Yeast Strain
For use with Clontech's Matchmaker Gold Yeast Two-Hybrid System
- Y2HGold Yeast Strain
Includes the sensitive Aureobasidin A antibiotic resistance gene as one of four reporters
- Matchmaker AD LD-Insert Screening Amplimer Set
PCR primers for amplifying and sequencing cDNA inserts in GAL4 Activation Domain (AD) Vectors
- Matchmaker Insert Check PCR Mix 2
2X pre-mix that allows quick and easy PCR directly from yeast colonies
Yeast Two-hybrid System 
- Antibodies & ELISA
- cDNA Synthesis
- Cell Biology
- Cloning
- Gene Function
- Fluorescent Proteins
- Next-gen Sequencing
- RNA-seq
- RNA-seq for Single Cell
- RNA-seq for Ultra-low Input
- RNA-seq Kits for Automation
- Stranded RNA-seq for Mammalian Samples
- Stranded RNA-seq for Non-Mammalian Samples
- Universal Low-input Total RNA-seq for Illumina or Ion Torrent
- Targeted RNA-seq for Illumina
- SMARTer RNA Unique Dual Index Kits
- Legacy RNA-seq Kits
- DNA-seq
- Whole-genome Amplification
- Immune Profiling
- Epigenetics and Small RNA Sequencing
- NGS Accessories
- RNA-seq
- Nucleic Acid Purification
- PCR
- Plant Tissue Culture
- Protein Research
- Accessory Enzymes
- Antibodies & Immunoprecipitation
- Expression Systems & Vectors
- Glycobiology
- Mass Spectrometry Reagents
- Protein Purification
- Protein Sequencing & Analysis
- SDS-PAGE & Western Blotting
- Yeast Hybrid Systems
- Real-Time PCR
- Stem Cell Research
- Viral Transduction
