AAV Vector Systems
AAV helper-free vector systems
Our helper-free AAV systems are designed for the preparation of high-titer AAV particles without the use of a helper virus. Each kit includes three plasmids encoding the factors necessary to prepare recombinant AAV particles by transfection of HEK293T cells. To prepare particles, co-transfect HEK293T cells with the three plasmids. Then, 2–3 days later extract particles using the included AAV Extraction Solution, a reagent that allows simple and high-yield AAV particle isolation while minimizing protein and nucleic acid contamination. In addition to offering AAV vectors for constitutive or inducible expression, we also provide AAVpro vectors for gene editing via CRISPR/Cas9 or Cre recombinase.
- Helper-free expression system (CMV promoter)
Preparation of AAV particles with the AAV2 serotype, without the use of a helper virus
- Tet-inducible Promoter
All-in-one pAAV-TetOne vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter, and your gene of interest from the PTRE3GS promoter
- AAV CRISPR/Cas9 Systems
Two kits simultaneously deliver expression cassettes for Cas9 and a user-defined single guide RNA (sgRNA) to mammalian cells
- AAV2-Cre Recombinase
Preparation of serotype 2 adeno-associated virus (AAV2) particles to deliver a Cre recombinase gene cassette to mammalian cells
- Beta-galactosidase System
Provides plasmids necessary for preparing AAV2 particles to deliver the lacZ reporter gene into mammalian cells
- ZsGreen1 Control Vector
AAV vector plasmid has inverted terminal repeats (ITRs) at both ends, function as origins of genomic replication and contribute to packaging of viral particles
AAV Vector Systems 
- Antibodies & ELISA
- cDNA Synthesis
- Cell Biology
- Cloning
- Gene Function
- Fluorescent Proteins
- Next-gen Sequencing
- RNA-seq
- RNA-seq for Single Cell
- RNA-seq for Ultra-low Input
- RNA-seq Kits for Automation
- Stranded RNA-seq for Mammalian Samples
- Stranded RNA-seq for Non-Mammalian Samples
- Universal Low-input Total RNA-seq for Illumina or Ion Torrent
- Targeted RNA-seq for Illumina
- SMARTer RNA Unique Dual Index Kits
- Legacy RNA-seq Kits
- DNA-seq
- Whole-genome Amplification
- Immune Profiling
- Epigenetics and Small RNA Sequencing
- NGS Accessories
- RNA-seq
- Nucleic Acid Purification
- PCR
- Plant Tissue Culture
- Protein Research
- Accessory Enzymes
- Antibodies & Immunoprecipitation
- Expression Systems & Vectors
- Glycobiology
- Mass Spectrometry Reagents
- Protein Purification
- Protein Sequencing & Analysis
- SDS-PAGE & Western Blotting
- Yeast Hybrid Systems
- Real-Time PCR
- Stem Cell Research
- Viral Transduction
