Secreated Alkaline Phosphatase Assays
The Great EscAPe SEAP kits
The Great EscAPe SEAP kits use a secreted alkaline phosphatase assay to analyze cis-acting DNA sequences and trans-acting factors. The kinetics of gene expression can easily be studied with this secreted alkaline phosphatase assay by repeatedly sampling the same culture. The kit has an extremely low background signal, with a wide dynamic range and high sensitivity. Furthermore, the robust signal is extremely stable over time. Cells can be studied further after the secreted alkaline phosphatase assay using northern blots, RNase protection assays, western blots, and other methods.
- Ready-To-Glow Dual Secreted Reporter Assay
Contains the buffers and substrates for use with the vectors contained in the Ready-To-Glow Dual Secreted Reporter Vector Kit
- pSEAP2 Vectors
Designed to enable the secreted form of human alkaline phosphatase (SEAP) to be used as a reporter gene
- SEAP Fluorescence Detection Kit
Reagents necessary for the detection of this enzyme activity using the fluorescent substrate 4-methylumbelliferyl phosphate (MUP)
- SEAP Chemiluminescence Kit 2.0
Complete system for detection of secreted alkaline phosphatase (SEAP) activity in the growth media from mammalian cells expressing this enzyme