Matchmaker Insert Check PCR Mix 2
Matchmaker Insert Check PCR Mix 2
Brand: Takara Bio.
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Matchmaker Insert Check PCR Mix 2
Matchmaker Insert Check PCR Mix 2 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix and perform 30 cycles of PCR. Matchmaker Insert Check PCR Mix 2 is designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems (Cat. Nos. 630491 and 630489, respectively) to amplify and screen cDNA inserts from prey/library plasmids following yeast one- and two-hybrid screening. The Mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7- Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.
Overview
- Quick, easy PCR amplification directly from yeast colonies
- Complete premix contains primers, dNTPs, and buffer—just add your cells and go!
- Primers are also available separately
Applications
- Yeast two-hybrid screening
- Yeast one-hybrid screening
Use colony PCR to quickly amplify and screen your positive candidate clones
Use colony PCR to quickly amplify and screen your positive candidate clones. Matchmaker Insert Check PCR Mix 2 was used to amplify inserts from 15 randomly selected colonies from candidate clones obtained with our Matchmaker Gold System (Lanes 2–16). The results allowed the clones to be quickly sorted for further analysis. Lane 1: No template control. Lane M1: 1 kb ladder. Lane M2: 100 bp ladder.
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Use colony PCR to quickly amplify and screen your positive candidate clones
Use colony PCR to quickly amplify and screen your positive candidate clones. Matchmaker Insert Check PCR Mix 2 was used to amplify inserts from 15 randomly selected colonies from candidate clones obtained with our Matchmaker Gold System (Lanes 2–16). The results allowed the clones to be quickly sorted for further analysis. Lane 1: No template control. Lane M1: 1 kb ladder. Lane M2: 100 bp ladder.
