pBridge Vector
This vector allows expression of two proteins: a DNA-binding domain fusion, and an additional protein under control of an inducible methionine promoter. In combination with an activation domain fusion and reporter strain from a GAL4-based two-hybrid system, one can establish a three hybrid system for the investigation of ternary protein complexes.
Overview
- The most popular and widely cited yeast two-hybrid vectors
- pGBKT7 and pGADT7 are components of the Matchmaker Gold Yeast Two-Hybrid System
- pBridge expresses a third protein for modified two-hybrid studies
Applications
- Yeast two-hybrid screening
- Yeast three-hybrid screening
pBridge Three-Hybrid Vector map
pBridge Three-Hybrid Vector map. A target protein is expressed as a fusion to the DNA-BD, while a second target protein is conditionally expressed from the Pmet25 promoter. Interactions dependent on three separate proteins can be investigated when pBridge is combined with an AD fusion vector from a GAL4-based two-hybrid system.
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pBridge Three-Hybrid Vector map
pBridge Three-Hybrid Vector map. A target protein is expressed as a fusion to the DNA-BD, while a second target protein is conditionally expressed from the Pmet25 promoter. Interactions dependent on three separate proteins can be investigated when pBridge is combined with an AD fusion vector from a GAL4-based two-hybrid system.
