GC-Rich PCR
Amplify templates that contain up to 90% GC content with ease
GC-rich templates, such as the 5' ends of many mammalian cDNAs, are difficult to amplify using conventional PCR techniques due the presence of secondary structures that resist denaturation and prevent efficient primer annealing. Takara Bio offers several DNA polymerases designed to handle such challenging targets.
- PrimeSTAR GXL DNA Polymerase
Our most robust high-fidelity polymerase available for challenging targets (GC-rich sequences, long amplicons, excess template)
- PrimeSTAR HS with GC buffer
GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions
- LA Taq DNA Polymerase with GC Buffers
Routine amplification of products up to 20 kb in length, including GC-rich amplicons
- Advantage GC 2 Polymerase
Includes proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two additives proven to enable amplification of templates with <90% GC content with minimal buffer optimization
- Advantage GC Genomic LA Polymerase Mix
Offer 6.5X higher fidelity than wild-type Taq DNA polymerase, and allow synthesis of products up to 48 kb
GC-Rich PCR 
- Antibodies & ELISA
- cDNA Synthesis
- Cell Biology
- Cloning
- Gene Function
- Fluorescent Proteins
- Next-gen Sequencing
- RNA-seq
- RNA-seq for Single Cell
- RNA-seq for Ultra-low Input
- RNA-seq Kits for Automation
- Stranded RNA-seq for Mammalian Samples
- Stranded RNA-seq for Non-Mammalian Samples
- Universal Low-input Total RNA-seq for Illumina or Ion Torrent
- Targeted RNA-seq for Illumina
- SMARTer RNA Unique Dual Index Kits
- Legacy RNA-seq Kits
- DNA-seq
- Whole-genome Amplification
- Immune Profiling
- Epigenetics and Small RNA Sequencing
- NGS Accessories
- RNA-seq
- Nucleic Acid Purification
- PCR
- Plant Tissue Culture
- Protein Research
- Accessory Enzymes
- Antibodies & Immunoprecipitation
- Expression Systems & Vectors
- Glycobiology
- Mass Spectrometry Reagents
- Protein Purification
- Protein Sequencing & Analysis
- SDS-PAGE & Western Blotting
- Yeast Hybrid Systems
- Real-Time PCR
- Stem Cell Research
- Viral Transduction
