Prey Rescue by Colony PCR
PCR amplification directly from yeast colonies
Matchmaker Insert Check PCR Mix 2 is a 2X premix that allows quick and easy PCR amplification directly from yeast colonies. It is a complete mix designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems to quickly amplify, sort, and analyze the cDNA inserts in prey/library plasmids from positive clones identified in library screens. The mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7-Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.
- Matchmaker Insert Check PCR Mix 2
2X pre-mix that allows quick and easy PCR directly from yeast colonies
- Matchmaker AD LD-Insert Screening Amplimer Set
PCR primers for amplifying and sequencing cDNA inserts in GAL4 Activation Domain (AD) Vectors
Prey Rescue by Colony PCR 
- Antibodies & ELISA
- cDNA Synthesis
- Cell Biology
- Cloning
- Gene Function
- Fluorescent Proteins
- Next-gen Sequencing
- RNA-seq
- RNA-seq for Single Cell
- RNA-seq for Ultra-low Input
- RNA-seq Kits for Automation
- Stranded RNA-seq for Mammalian Samples
- Stranded RNA-seq for Non-Mammalian Samples
- Universal Low-input Total RNA-seq for Illumina or Ion Torrent
- Targeted RNA-seq for Illumina
- SMARTer RNA Unique Dual Index Kits
- Legacy RNA-seq Kits
- DNA-seq
- Whole-genome Amplification
- Immune Profiling
- Epigenetics and Small RNA Sequencing
- NGS Accessories
- RNA-seq
- Nucleic Acid Purification
- PCR
- Plant Tissue Culture
- Protein Research
- Accessory Enzymes
- Antibodies & Immunoprecipitation
- Expression Systems & Vectors
- Glycobiology
- Mass Spectrometry Reagents
- Protein Purification
- Protein Sequencing & Analysis
- SDS-PAGE & Western Blotting
- Yeast Hybrid Systems
- Real-Time PCR
- Stem Cell Research
- Viral Transduction
