Prey Rescue by Colony PCR
PCR amplification directly from yeast colonies
Matchmaker Insert Check PCR Mix 2 is a 2X premix that allows quick and easy PCR amplification directly from yeast colonies. It is a complete mix designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems to quickly amplify, sort, and analyze the cDNA inserts in prey/library plasmids from positive clones identified in library screens. The mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7-Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.