Direct PCR amplification from tissues or crude extracts, without the need for DNA purification
Direct PCR allows for amplification directly from tissues or crude extracts, including from plants and animals, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces costs. Take advantage of polymerases that are robust enough to overcome PCR inhibitors.
Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited, and is well-suited for high-throughput applications.