DNA-seq for FFPE and Cell-free DNA
Complete, fast, and accurate sequencing from challenging samples
Unlike other NGS library preparation kits, which are based on ligation of Y-adapters, our patented ThruPLEX HV technology uses stem-loop adapters to construct high-quality libraries in a fast, efficient workflow. This protocol is the simplest in the industry, and enables the addition of adapters containing Illumina-compatible UDIs and UMIs in three short steps. The sample never leaves the tube, ensuring accurate sample tracking, minimizing handling errors, and preventing loss of valuable samples. Sensitive, even coverage is one of the hallmarks of this system, with both DNA-seq and tag-seq kits providing consistent, reproducible coverage for a broad range of GC content, without bias.
ThruPLEX DNA-Seq HV PLUS outperforms KAPA HyperPLUS and NEBNext Ultra II FS in coverage of GC-rich regions, and offers efficiency, simplicity, and reliability in a single-tube workflow....