Traditional Matchmaker Libraries
Traditional Matchmaker Libraries
Brand: Takara Bio.
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Traditional Matchmaker Libraries
These libraries were constructed in either the pACT, pACT2, or pGAD-GH vectors, which express the GAL4 activation domain fused to high-complexity, tissue-specific cDNA. These libraries are compatible with Matchmaker yeast strains Y2H Gold, AH109, and Y187, or any previous Matchmaker GAL4 strain.
Traditional libraries are supplied in E. coli, and therefore must be amplified and transformed into yeast before use. For transformations, we recommend the Yeastmaker Yeast Transformation System 2.
We now recommmend using a yeast two-hybrid library Mate & Plate or normalized yeast two-hybrid Mate & Plate library with the Matchmaker Gold System.
Overview
- High-complexity, GAL4 yeast two-hybrid libraries
- Compatible with Matchmaker yeast strains Y2H Gold, AH109, and Y187
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