Site-specific gene targeting in virtually any organism

We offer a complete line of products for CRISPR/Cas9-based gene editing experiments. This product line includes in vitro transcription and screening kits for testing the efficiency of candidate guide RNAs before delivery to target cells, an effective recombinant Cas9 nuclease ready for electroporation, many Cas9/sgRNA delivery options for a wide variety of cell lines, and mutation detection and genotype confirmation kits for quick identification of gene editing results.

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  1. Guide-it Recombinant Cas9 (Electroporation-Ready)

    Recombinant wild-type Streptococcus pyogenes Cas9 nuclease expressed with a C-terminal nuclear localization signal (NLS)

  2. TransIT-siQUEST Transfection Reagent

    A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells

  3. Long ssDNA for Knockins

    On-demand production of long single-stranded DNA (ssDNA) up to 5,000 nt in length for use as a repair template in knockin experiments

  4. Guide-it Knockin Screening Kit

    Sensitive detection of successful homologous recombination (HR) events in mixed or clonal cell populations edited using CRISPR/Cas9 system

  5. Genome-wide sgRNA Library System

    Lentiviral-based, genome-wide CRISPR/Cas9 knockout screens in human cells

  6. Guide-it Mutation Detection Kit

    PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases

  7. In vitro Transcription and Screening Kits for sgRNA

    Kits for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies

  8. Cas9-sgRNA Gesicle Production

    Cloning and expressing your target-specific guide RNA, plus packaging reagents for producing CRISPR/Cas9 gesicles

  9. Cas9 Antibodies

    Monoclonal and polyclonal antibodies raised against recombinant Cas9 protein from Streptococcus pyogenes

  10. CRISPR/Cas9 Plasmid Systems

    Complete system for cloning and expression of a target single guide RNA (sgRNA) for mammalian gene modification

  11. Lentiviral CRISPR/Cas9 System

    Complete systems for lentiviral-mediated CRISPR/Cas9 genome editing

  12. AAV CRISPR/Cas9 Systems

    Two kits simultaneously deliver expression cassettes for Cas9 and a user-defined single guide RNA (sgRNA) to mammalian cells

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