Bicistronic IRES Vectors
Simultaneous expression of two proteins separately
The IRES of the encephalomyocarditis virus (ECMV) permits the translation of two open reading frames from one messenger RNA. Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location. Thus, two proteins are expressed simultaneously from the same bicistronic mRNA transcript.
Our pIRES vectors contain two multiple cloning sites flanking the central IRES for you to clone and express two genes of interest.