Make Your Own Mate & Plate Library System
Make Your Own Mate & Plate Library System
Brand: Takara Bio.
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Make Your Own Mate & Plate Library System
Mate & Plate Libraries are by far the easiest libraries to screen for protein-protein interactions using a GAL4 yeast two-hybrid system. Several Mate & Plate Libraries are available ready-made from Takara Bio. For libraries that are not available, this system provides the necessary components and a simple, highly efficient method to make your own Mate & Plate Library using SMART technology and the highly efficient homologous recombination machinery of Saccharomyces cerevisiae.
Overview
- Library construction occurs directly in yeast using SMART technology
- No laborious cloning or library amplification steps
- Material for hundreds of yeast two-hybrid screens
Applications
- Yeast two-hybrid screening
Library generation using n vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec.
Library generation using in vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec. The homologues are generated by SMART cDNA synthesis. Colonies are pooled, mixed, and aliquoted into multiple vials. Each single 1 ml vial can be used for a two-hybrid screen.
Mate & Plate libraries display broad insert representation
Mate & Plate libraries display broad insert representation. A human bone marrow library was made using the Make Your Own “Mate & Plate” Library System. Inserts from 15 randomly picked colonies were analyzed by yeast colony PCR using the Advantage 2 Polymerase Mix (Cat. No. 639201), and the Matchmaker AD LD-Insert Screening Amplimer Set (Cat. No. 630433). As seen in Lanes 1–15, every colony contained an insert of a different size. Lane M: 1 kb DNA ladder molecular weight marker.
SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec
SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec.
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Library generation using n vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec.
Library generation using in vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec. The homologues are generated by SMART cDNA synthesis. Colonies are pooled, mixed, and aliquoted into multiple vials. Each single 1 ml vial can be used for a two-hybrid screen.
Mate & Plate libraries display broad insert representation
Mate & Plate libraries display broad insert representation. A human bone marrow library was made using the Make Your Own “Mate & Plate” Library System. Inserts from 15 randomly picked colonies were analyzed by yeast colony PCR using the Advantage 2 Polymerase Mix (Cat. No. 639201), and the Matchmaker AD LD-Insert Screening Amplimer Set (Cat. No. 630433). As seen in Lanes 1–15, every colony contained an insert of a different size. Lane M: 1 kb DNA ladder molecular weight marker.
SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec
SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec.
