Ligation-free Cloning
In-Fusion Cloning
The In-Fusion Cloning products allow ligation-independent cloning of PCR products into any vector, at any site of linearization. The In-Fusion Cloning reaction, which takes as little as 15 minutes, is specific and directional, ensuring an exceptionally high rate of cloning accuracy in all applications. The liquid format, In-Fusion HD Cloning Plus system, provides the ability to customize reaction volumes and plasticware, while the lyophilized format, In-Fusion HD EcoDry Cloning Plus system, version offers prealiquoted components to minimize handling errors, and can be stored at room temperature.
- In-Fusion Snap Assembly Master Mix
Designed for fast, directional cloning of one or more fragments of DNA into any vector
- In-Fusion HD Cloning Plus
Optimised, complete cloning system includes cloning enzyme mix, PCR enzyme, competent cells, and PCR clean-up & gel extraction kit
- In-Fusion HD EcoDry Cloning Plus
Optimised, complete cloning system includes lyophilised cloning enzyme mix, PCR enzyme, competent cells, and gel extraction kit
- Cloning Enhancer
Removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning
Ligation-independent, directional cloning of PCR products into any vector, at any site. Clear winner over NEBuilder HiFi in overall efficiency, yielding 16x more colonies and 95% accuracy....
Ligation-free Cloning 
- Antibodies & ELISA
- cDNA Synthesis
- Cell Biology
- Cloning
- Gene Function
- Fluorescent Proteins
- Next-gen Sequencing
- RNA-seq
- RNA-seq for Single Cell
- RNA-seq for Ultra-low Input
- RNA-seq Kits for Automation
- Stranded RNA-seq for Mammalian Samples
- Stranded RNA-seq for Non-Mammalian Samples
- Universal Low-input Total RNA-seq for Illumina or Ion Torrent
- Targeted RNA-seq for Illumina
- SMARTer RNA Unique Dual Index Kits
- Legacy RNA-seq Kits
- DNA-seq
- Whole-genome Amplification
- Immune Profiling
- Epigenetics and Small RNA Sequencing
- NGS Accessories
- RNA-seq
- Nucleic Acid Purification
- PCR
- Plant Tissue Culture
- Protein Research
- Accessory Enzymes
- Antibodies & Immunoprecipitation
- Expression Systems & Vectors
- Glycobiology
- Mass Spectrometry Reagents
- Protein Purification
- Protein Sequencing & Analysis
- SDS-PAGE & Western Blotting
- Yeast Hybrid Systems
- Real-Time PCR
- Stem Cell Research
- Viral Transduction
