Modifying Enzymes
- Alkaline Phosphatase (Shrimp)
SAP is irreversibly heat inactivated at 65°C for 15 minutes, allowing for streamlining of cloning experiments
- Alkaline Phosphatase (E coli C75)
Catalyses the removal of most phosphomonoester bonds from the 5' and 3' termini of DNA and RNA without degrading diphosphate or triphosphate linkages
- Alkaline Phosphatase (Calf Intestine)
Catalyses the hydrolysis of 5'-phosphate termini from DNA, RNA, dNTPs and rNTPs
- S1 Nuclease
Degrades single-stranded nucleic acids, including the single-stranded regions of duplex DNA, RNA, or DNA/RNA
- Ribonuclease H (RNase H)
Hydrolyses single-stranded RNA via a magnesium or manganese cofactor
- Recombinant DNase I (RNase-free)
Nonspecifically catalyzes the degradation of both single- and double-stranded DNA and DNA-RNA hybrids
- Mung Bean Nuclease
Hydrolyse single-stranded DNA and RNA into 5'-phosphate- and 3'-hydroxyl-containing products
- Micrococcal Nuclease
Endonuclease that preferentially digests single-stranded DNA and RNA, especially at AT or AU rich regions
- Exonuclease III
3' to 5' exonuclease that removes mononucleotides from the 3'-hydroxyl termini of double-stranded DNA or DNA-RNA hybrids
- Exonuclease I
3' to 5' exonuclease for single-stranded DNA degradation, resulting in the production of 5'-phosphate mononucleotides
- Cryonase Cold-Active Nuclease
Digest all types of DNA and RNA (single-stranded, double-stranded, linear or circularised), even on ice
- Yatalase Enzyme
Used to lyse the cell walls of filamentous fungi