Matchmaker Gold Yeast Two-Hybrid System
Matchmaker Gold Yeast Two-Hybrid System
Brand: Takara Bio.
In stock
SKU
Matchmaker Gold Yeast Two-Hybrid System
Complete GAL4-based two-hybrid system to detect protein interactions
Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.
Overview
- Very low incidence of false positives
- Four reporters, including Aureobasidin A antibiotic resistance
- Simple mating protocol
- Broad range of compatible “Mate & Plate” libraries
Applications
- Yeast two-hybrid screening
The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.
Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency. Interacting bait and prey fusion proteins drive the expression of four different reporters from three different GAL4-responsive promoters (M1, G1, and G2), which are stably integrated in the genome of the reporter strain, Y2H Gold. Aureobasidin A (AbA) resistance and the two auxotrophic reporters for histidine and adenine biosynthesis confer growth selection in the presence of AbA and on histidine- and adenine-deficient media, while the a-galactosidase reporter produces blue colonies in the presence of X-alpha-Gal.
Chien, C. T., Bartel, P. L., Sternglanz, R. & Fields, S. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Nat. Acad. Sci. U.S.A. 88, 9578–9582 (1991).
Fields, S. & Song, O. A novel genetic system to detect protein-protein interactions. Nature 340, 245–247 (1989).
Write Your Own Review
The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.
Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency. Interacting bait and prey fusion proteins drive the expression of four different reporters from three different GAL4-responsive promoters (M1, G1, and G2), which are stably integrated in the genome of the reporter strain, Y2H Gold. Aureobasidin A (AbA) resistance and the two auxotrophic reporters for histidine and adenine biosynthesis confer growth selection in the presence of AbA and on histidine- and adenine-deficient media, while the a-galactosidase reporter produces blue colonies in the presence of X-alpha-Gal.
