S1 Nuclease
S1 Nuclease
S1 Nuclease is an endonuclease that specifically degrades single-stranded nucleic acids, including the single-stranded regions of duplex DNA, RNA, or DNA/RNA. S1 Nuclease preferentially targets DNA over RNA. This endonuclease can also introduce single-stranded nicks and breaks in duplex DNA, RNA, and DNA/RNA. S1 Nuclease is supplied in a buffer of 10 mM sodium acetate (pH 4.6), 150 mM NaCl, 0.05 mM ZnSO4 and 50% glycerol.
Applications
- Nuclease protection assays
- Double-stranded DNA end blunting
- RNA transcript mapping
- cDNA hairpin removal
Source
Aspergillus oryzae
Storage
–20°C
Buffer
Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 4.6), 2.8 M NaCl and 10 mM ZnSO4
Unit definition
One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into an acid-soluble product in 1 minute at 37°C and pH 4.6.
Concentration
100–200 U/µl
Product citations
Ando, T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim. Biophys. Acta 114, 158–68 (1966).
Berk, A. J. & Sharp, P. A. Spliced early mRNAs of simian virus 40. Proc. Natl. Acad. Sci. U. S. A. 75, 1274–8 (1978).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor laboratory press. New York (1989). doi:574.873224 1/1989
Wiegand, R. C., Godson, G. N. & Radding, C. M. Specificity of the S1 nuclease from Aspergillus oryzae. J. Biol. Chem. 250, 8848–55 (1975).
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