Ribonuclease H (RNase H)
Ribonuclease H (RNase H)
Brand: Takara Bio.
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Ribonuclease H (RNase H)
Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation. Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.
Applications
- Removal of RNA template following second-strand cDNA synthesis
- Removal of mRNA poly(A) sequence
- Reverse transcription
Source
Recombinant E. coli
Storage
–20°C
Unit definition
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.
Concentration
20–60 U/µl
Schultz, S. J. & Champoux, J. J. RNase H activity: structure, specificity, and function in reverse transcription. Virus Res. 134, 86–103 (2008).
Vournakis, J. N., Efstratiadis, A. & Kafatos, F. C. Electrophoretic patterns of deadenylylated chorion and globin mRNAs. Proc. Natl. Acad. Sci. U. S. A. 72, 2959–63 (1975).
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