Yatalase Enzyme
Yatalase Enzyme
Yatalase is an enzyme commonly used for filamentous fungi cell wall digestion, enabling protoplast preparation. The product is prepared from cell-culture supernatants of Corynebacterium sp. OZ-21 and consists mainly of chitinase, chitobiase, and beta-1,3-glucanase.
Overview
- Can be used to prepare protoplasts from filamentous fungi
- Excellent thermostability at room temperature
- Efficiently digests native chitin
Applications
Cell-wall digestion of filamentous fungi
Properties
- Optimum pH: 7.2
- Optimum temperature for enzyme activity: 37°C
Enzyme activities found in Yatalase
Yields of protoplasts
Product citations
Iwashita, K., Todoroki, K., Kimura, H., Shimoi, H. & Ito, K. Purification and characterization of extracellular and cell wall bound beta-glucosidases from Aspergillus kawachii. Biosci. Biotechnol. Biochem. 62, 1938–46 (1998).
Tsuchiya, K., Nishimura, K. & Iwahara, M. Purification and Characterization of Glutamate Decarboxylase from Aspergillus oryzae. Food Sci. Technol. Res 9, 283–287 (2003).