Mung Bean Nuclease
Mung Bean Nuclease
Brand: Takara Bio.
In stock
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Mung Bean Nuclease
Mung Bean Nuclease
Mung Bean Nuclease acts as a preferential DNA and RNA endonuclease, hydrolyzing single-stranded DNA and RNA into 5'-phosphate- and 3'-hydroxyl-containing products. If used at very high concentrations, Mung Bean Nuclease is also capable of degrading double-stranded DNA, RNA or DNA/RNA fragments from both ends. Due to its single-stranded DNA and RNA endonuclease activity, Mung Bean Nuclease is often used during transcript mapping, duplex or hybrid DNA and RNA blunt ending, and cDNA preparation.
Applications
- Double-stranded DNA, RNA, or DNA/RNA blunt ending
- cDNA hairpin loop cleavage
Buffer
Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 5.0), 1000 mM NaCl, 10 mM zinc acetate, and 50% glycerol
Product citations
Kowalski, D., Kroeker, W. D. & Laskowski, M. Mung bean nuclease I. Physical, chemical, and catalytic properties. Biochemistry 15, 4457–63 (1976).
Kroeker, W. D., Kowalski, D. & Laskowski, M. Mung bean nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry 15, 4463–7 (1976).
Mathis, D. J., Elkaim, R., Kédinger, C., Sassone-Corsi, P. & Chambon, P. Specific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units. Proc. Natl. Acad. Sci. U. S. A. 78, 7383–7 (1981).
Sheflin, L. G. & Kowalski, D. Altered DNA conformations detected by mung bean nuclease occur in promoter and terminator regions of supercoiled pBR322 DNA. Nucleic Acids Res. 13, 6137–54 (1985).
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