Recombinant DNase I (RNase-free)
Recombinant DNase I (RNase-free)
Brand: Takara Bio.
In stock
SKU
Recombinant DNase I (RNase-free)
DNase I (RNase-free)
DNase I (RNase-free) is a recombinant endonuclease that nonspecifically catalyzes the degradation of both single- and double-stranded DNA and DNA-RNA hybrids, producing 5'-phosphate and 3'-hydroxyl termini-containing oligonucleotides of varying lengths. The protease activity of DNase I endonuclease has been eliminated; therefore, this enzyme is stable at its optimal (neutral) pH range and is suitable for RNA preparation at neutral pH.
Applications
- Template DNA digestion following in vitro transcription
- Genomic DNA digestion prior to RT-PCR
- Nick translation with DNA polymerase
- Shotgun DNA library construction
- Footprinting analysis
Components
Supplied Buffer (10X): 400 mM Tris-HCl, pH7.5; 80 mM MgCl2; 50 mM DTT
Source
Recombinant non-animal host containing bovine pancreas DNase I-encoding plasmid
Storage
–20°C
Unit definition
One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 25°C (pH 5.0) using calf thymus DNA as the substrate (Kunitz unit).
Concentration
5 U/µl
References
Anderson, S. Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Res. 9, 3015–27 (1981).
Galas, D. J. & Schmitz, A. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 5, 3157–70 (1978).
Green, M. R., Maniatis, T. & Melton, D. A. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32, 681–94 (1983).
Kunitz, M. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity. J. Gen. Physiol. 33, 349–62 (1950).
Rigby, P. W., Dieckmann, M., Rhodes, C. & Berg, P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113, 237–51 (1977).
Write Your Own Review
