Alkaline Phosphatase (E coli C75)
Alkaline Phosphatase (E coli C75)
Brand: Takara Bio.
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Alkaline Phosphatase (E coli C75)
Bacterial alkaline phosphatase (E. coli)
Bacterial alkaline phosphatase (E. coli) nonspecifically catalyzes the removal of most phosphomonoester bonds from the 5' and 3' termini of DNA and RNA without degrading diphosphate or triphosphate linkages. The enzyme is purified from an E. coli strain that does not harbor RNase activity. Bacterial alkaline phosphatase (E. coli) is supplied in a buffer of 50 mM Tris-HCl (pH 8.0), 100 mM KCl, 1 mM MgCl2, and 50% glycerol.
Applications
- Phosphate group removal to reduce self-ligation and vector background
- Dephosphorylation of DNA 5' termini prior to kinase labeling reactions
Source
E. coli strain C75
Storage
–20°C
Note
Bacterial alkaline phosphatase (E. coli) is extremely stable and is not completely heat-inactivated; therefore, to inactivate the enzyme, reaction products need to be purified twice via phenol-chloroform extraction followed by ethanol precipitation.
Buffer
Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2]
Unit definition
One unit is defined as the amount of enzyme required to generate 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 25°C and pH 8.0.
Concentration
0.5 U/µl
Product citations
Reid, T. W. & Wilson, I. B. E. coli Alkaline Phosphatase. Enzym. 4, 373–415 (1971).
Takanami, M. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids: I. The 5'-termini of Escherichia coli ribosomal RNA. J. Mol. Biol. 23, 135–48 (1967).
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