Alkaline Phosphatase (Calf Intestine)
Alkaline Phosphatase (Calf Intestine)
Brand: Takara Bio.
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Alkaline Phosphatase (Calf Intestine)
Calf intestinal alkaline phosphatase (CIAP)
Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5'-phosphate termini from DNA, RNA, dNTPs and rNTPs. This enzyme can be used to reduce linear vector self-ligation and vector background during cloning. CIAP is supplied in a buffer of 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2, and 50% glycerol.
Applications
- Reduction of linear vector self-ligation and vector background during cloning
- Dephosphorylation of DNA prior to kinase labeling reactions
- Template preparation prior to 5'-end labeling
- Protein dephosphorylation
Source
Yeast
Storage
–20°C
Notes
Calf intestinal alkaline phosphatase (CIAP) cannot be completely heat inactivated, and therefore it is recommended that the DNA product be purified by gel or spin column purification or phenol-chloroform extraction.
Buffer
Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].
Unit definition
One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.
Concentration
10–30 U/µl
Product citations
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).
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