NucleoZOL
Applications
- RNA isolation from cultured / bacterial / yeast cells, human / animal / plant tissue, (viral) fluids
Features
- No chloroform, no phase separation: easy and safe procedure
- High RNA yield and separation of small and large RNA possible
- Combination with NucleoSpin® RNA Columns possible
Specifications
- Technology: Liquid one phase extraction
- Fragment size: Small RNA: 10–200 nt, large RNA: > 200 nt
- Typical yield (total RNA): 1–8 μg RNA/mg sample
- A260/A280: 1.8–2.1
- Elution volume: Flexible
- Processing time: < 1 h
Summary of advantages of NucleoZOL compared to competitor Zol
The table indicates the advantages of NucleoZOL procedure compared to typical competitor Zol products. The NucleoZOL procedure offers an easy and much more convenient liquid handling. A laborious chloroform two-phase separation is not necessary. Additionally, NucleoZOL can be combined with the proven NucleoSpin® technology.
High RNA quality from fibrous tissue
Total RNA was isolated with NucleoZOL from 60 mg mouse heart tissue. RNA was analyzed on an Agilent Bioanalyzer. The RIN of 9 indicates perfect RNA quality.
Market-leading RNA yields
RNA was extracted from different starting materials. RNA was quantified by qRT-PCR and relative yields were calculated (NucleoZOL=100 %). RNA isolation with NucleoZOL results in similar or better RNA yields compared to standard two-phase extraction methods (competitor Zol).
Low DNA contamination with NucleoZOL
DNA contamination was quantified by qPCR (competitor Zol=100 %). Compared to a standard two-phase extraction, only a minimum of DNA is carried over during purification with NucleoZOL.
High superior RNA recovery and easy handling
Total RNA was isolated with NucleoZOL combined with mini spin columns (NucleoSpin® RNA Set for NucleoZOL) or with a competitor product, that combines a liquid extraction with a columnbased method (competitor Z). Total RNA yields were quantified using an Agilent 2100 Bioanalyzer™.
Procedure
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Summary of advantages of NucleoZOL compared to competitor Zol
The table indicates the advantages of NucleoZOL procedure compared to typical competitor Zol products. The NucleoZOL procedure offers an easy and much more convenient liquid handling. A laborious chloroform two-phase separation is not necessary. Additionally, NucleoZOL can be combined with the proven NucleoSpin® technology.
High RNA quality from fibrous tissue
Total RNA was isolated with NucleoZOL from 60 mg mouse heart tissue. RNA was analyzed on an Agilent Bioanalyzer. The RIN of 9 indicates perfect RNA quality.
Market-leading RNA yields
RNA was extracted from different starting materials. RNA was quantified by qRT-PCR and relative yields were calculated (NucleoZOL=100 %). RNA isolation with NucleoZOL results in similar or better RNA yields compared to standard two-phase extraction methods (competitor Zol).
Low DNA contamination with NucleoZOL
DNA contamination was quantified by qPCR (competitor Zol=100 %). Compared to a standard two-phase extraction, only a minimum of DNA is carried over during purification with NucleoZOL.
High superior RNA recovery and easy handling
Total RNA was isolated with NucleoZOL combined with mini spin columns (NucleoSpin® RNA Set for NucleoZOL) or with a competitor product, that combines a liquid extraction with a columnbased method (competitor Z). Total RNA yields were quantified using an Agilent 2100 Bioanalyzer™.
Procedure
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