Applications

• Small and large RNA isolation from cultured / bacterial / yeast cells, human / animal / plant tissue, reaction mixtures

Features

• Excellent RNA recovery and purity by chaotropic salt lysis without phenol / chloroform (patent pending)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Separation of small and large RNA possible

Specifications

• Technology: Silica membrane technology
• Sample material: Cells (< 10⁷), human / animal tissue (< 30 mg), plant tissue (< 50 mg), reaction mixtures (< 150 μL)
• Fragment size: ≥ 18 nt
• Typical yield: HeLa cells (10⁷): 10 μg small RNA, 95 μg large RNA
• Elution volume: 30–100 μL
• Binding capacity: 200 μg
• Processing time: < 45 min/6 preps total RNA)