NucleoSpin RNA Plus
NucleoSpin RNA Plus
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoSpin RNA Plus
Applications
- RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Features
- NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
- Convenient handling – lysate clearing and gDNA removal with one column in one step
- New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
Specifications
- Technology: Silica membrane technology (1. column for DNA removal and lysate clearing, 2. column for RNA isolation)
- A260/A280: 1.9–2.1
- Sample material: Cultured cells (< 10⁷), bacterial cells (< 10⁹), yeast cells (< 10⁸), human / animal tissue (< 30 mg)
- Fragment size: > 200 nt
- Typical yield: 40–100 μg
- Elution volume: 30–120 μL
- Binding capacity: 200 μg
- Processing time: 20 min/6 preps
RNA purification using NucleoSpin RNA Plus compared to a standard kit
RNA purification using NucleoSpin RNA Plus compared to a standard kit.
Efficient RNA recovery with the NucleoSpin RNA Plus kit
Efficient RNA recovery with the NucleoSpin RNA Plus kit. RNA was isolated from 5 x 106 HeLa cells and 20 mg mouse kidney using the NucleoSpin RNA Plus kit (blue) and a competitor kit (red). RNA recovery was quantified by qRT-PCR. Lower CT values indicate higher RNA yields.
Reliable genomic DNA removal with the NucleoSpin RNA Plus kit
Reliable genomic DNA removal with the NucleoSpin RNA Plus kit. NucleoSpin RNA Plus (MN; blue) and a competitor kit (Q; red) were used to isolate RNA from 5 x 106 HeLa cells and 20 mg mouse kidney. Residual genomic DNA was measured by qPCR. NucleoSpin RNA Plus showed higher CT values, indicating more efficient genomic DNA removal. Higher CT values mean less genomic DNA.
Isolate high-integrity RNA with the NucleoSpin RNA Plus kit
Isolate high-integrity RNA with the NucleoSpin RNA Plus kit. NucleoSpin RNA Plus (MN; blue) and a competitor kit (Q; red) were used to isolate RNA from 10 mg mouse kidney. Equal amounts of RNA from each prep were analyzed on an Agilent Bioanalyzer. The RIN value is higher for the MN kit, indicating higher RNA integrity.
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RNA purification using NucleoSpin RNA Plus compared to a standard kit
RNA purification using NucleoSpin RNA Plus compared to a standard kit.
Efficient RNA recovery with the NucleoSpin RNA Plus kit
Efficient RNA recovery with the NucleoSpin RNA Plus kit. RNA was isolated from 5 x 106 HeLa cells and 20 mg mouse kidney using the NucleoSpin RNA Plus kit (blue) and a competitor kit (red). RNA recovery was quantified by qRT-PCR. Lower CT values indicate higher RNA yields.
Reliable genomic DNA removal with the NucleoSpin RNA Plus kit
Reliable genomic DNA removal with the NucleoSpin RNA Plus kit. NucleoSpin RNA Plus (MN; blue) and a competitor kit (Q; red) were used to isolate RNA from 5 x 106 HeLa cells and 20 mg mouse kidney. Residual genomic DNA was measured by qPCR. NucleoSpin RNA Plus showed higher CT values, indicating more efficient genomic DNA removal. Higher CT values mean less genomic DNA.
Isolate high-integrity RNA with the NucleoSpin RNA Plus kit
Isolate high-integrity RNA with the NucleoSpin RNA Plus kit. NucleoSpin RNA Plus (MN; blue) and a competitor kit (Q; red) were used to isolate RNA from 10 mg mouse kidney. Equal amounts of RNA from each prep were analyzed on an Agilent Bioanalyzer. The RIN value is higher for the MN kit, indicating higher RNA integrity.
