Applications

• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Features

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready to use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set

Specifications

• Technology: Silica membrane technology
• Fragment size: > 200 nt
• A260/A280: 1.9–2.1
• Processing: Centrifugation
• Sample material: Cultured cells (< 5 x 10⁶) bacterial cells (< 10⁹), yeast cells (< 10⁸) Human / animal tissue (< 30 mg)
• Typical yield: HeLa cells (10⁶): 14 μg, bacterial cells (10⁹): 70 μg
• Elution volume: 30–120 μL
• Binding capacity: 200 μg
• Processing time: 35 min/6 preps