Applications

• Parallel isolation of RNA, DNA, and protein from undivided samples from cultured / bacteria / yeast cells, human / animal / plant tissue

Features

• Convenient one column preparation of RNA, DNA, and protein
• Easy and accurate protein quantification using the MACHEREY-NAGEL Protein Quantification Assay (page 56)
• Complete kit including NucleoSpin® Filters (shredders) for efficient lysis, rDNase for on-column DNA digestion, and Protein Solving Buffer

Specifications

• Technology: Silica membrane technology
• Sample material: Cells (< 5 x 10⁶), human / animal tissue (< 30 mg), plant tissue (< 100 mg)
• Fragment size: RNA: > 200 nt, DNA: < 30 kbp, protein: 15–300 kDa
• Typical yield: RNA: < 70 μg, DNA: < 6 μg, protein: < 1200 μg
• A260/A280: RNA: 1.9–2.1, DNA: 1.7–1.9
• Elution volume: RNA: 40–120 μL, DNA: 100 μL, protein: 10–100 μL
• Binding capacity: RNA: 200 μg, DNA: 10 μg*
• Processing time: RNA: 30 min/6 preps, RNA + DNA: 45 min/6 preps, RNA + DNA + protein: 35 min/6 preps