In vitro Transcription and Screening Kits for sgRNA
In vitro Transcription and Screening Kits for sgRNA
Brand: Takara Bio.
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In vitro Transcription and Screening Kits for sgRNA
Our Guide-it in vitro transcription and screening kits for sgRNA include four kits for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies. Each kit provides enough materials for 50 reactions. The Guide-it sgRNA In Vitro Transcription Kit (Cat. # 632635) is used to produce high yields of sgRNAs via in vitro transcription. This kit also includes the Guide-it IVT RNA Clean-Up Kit (Cat. # 632638), which is used to quickly purify sgRNA following in vitro transcription (IVT) reactions. Purified sgRNA from this kit is ready for direct use in transfection, electroporation, or in vitro cleavage assays. The Guide-it sgRNA Screening Kit (Cat. # 632639) enables you to test the efficacy of different single guide RNAs (sgRNAs) in vitro prior to using them in studies involving Cas9-mediated gene editing so that you don't waste time delivering ineffective sgRNAs to your cells. With this kit, a template containing a sgRNA target site is created by PCR, then combined with a sgRNA that you wish to test and recombinant Cas9 nuclease. The efficiency with which Cas9 nuclease cleaves the template can be measured using agarose gel electrophoresis. The Guide-it Complete sgRNA Screening System (Cat. # 632636) includes all of the kit components described above and is used to synthesize and test the efficacy of single guide RNAs (sgRNAs). The kit contains all the reagents needed to in vitro transcribe and purify sgRNAs, and test their efficacy against a PCR target fragment using recombinant Cas9 nuclease.
Overview
- Four kits for simple production, cleanup, and evaluation of the efficacy of sgRNA (single guide RNA) for Cas9-mediated gene editing studies
- In vitro transcribe and purify high yields of sgRNA with the Guide-it sgRNA In Vitro Transcription Kit (Cat. # 632635; includes the Guide-it IVT RNA Clean-Up Kit, Cat. # 632638) for transfection or in vitro screening
- Use the Guide-it IVT RNA Clean-Up Kit (Cat. # 632638) to quickly purify sgRNA after IVT reactions, for direct use in transfection, electroporation, or in vitro cleavage assays
- High-quality recombinant Cas9 protein included in the Guide-it sgRNA Screening Kit (Cat. # 632639)
- Best value: the Guide-it Complete sgRNA Screening System (Cat. # 632636) is a combination of both the Guide-it sgRNA In Vitro Transcription Kit (Cat. # 632635) and Guide-it sgRNA Screening Kit (Cat. # 632639), allowing you to both produce sgRNA and test its ability to cleave its putative target in vitro
Production of sgRNA by in vitro transcription and testing for cleavage using recombinant Cas9 protein
Schematic highlighting the key steps for using Guide-it kits for the production of high amounts of single guide RNA (sgRNA) and screening for target site cleavage efficacy using recombinant Cas9 protein.
sgRNA yield and quality for different gene targets using the Guide-it Complete sgRNA Screening System
sgRNA yield and quality for different gene targets using the Guide-it Complete sgRNA Screening System. Panel A. 20 µl of each sgRNA in vitro transcription (IVT) reaction was incubated for 4 hr at 37°C. The yield for each transcribed sgRNA was quantified using a NanoDrop spectrophotometer. Panel B. Each sgRNA IVT reaction was a run on an Agilent Bioanalyzer to assay for sample quality.
Flow chart describing how the Guide-it sgRNA In Vitro Transcription Kit (including the Guide-it IVT RNA Clean-Up Kit) and Guide-it sgRNA Screening Kit work together in the Guide-it Complete sgRNA Screening System, which can be used to synthesize and test the efficacy of sgRNAs
Flow chart describing how the Guide-it sgRNA In Vitro Transcription Kit (including the Guide-it IVT RNA Clean-Up Kit) and Guide-it sgRNA Screening Kit work together in the Guide-it Complete sgRNA Screening System, which can be used to synthesize and test the efficacy of sgRNAs.
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Production of sgRNA by in vitro transcription and testing for cleavage using recombinant Cas9 protein
Schematic highlighting the key steps for using Guide-it kits for the production of high amounts of single guide RNA (sgRNA) and screening for target site cleavage efficacy using recombinant Cas9 protein.
sgRNA yield and quality for different gene targets using the Guide-it Complete sgRNA Screening System
sgRNA yield and quality for different gene targets using the Guide-it Complete sgRNA Screening System. Panel A. 20 µl of each sgRNA in vitro transcription (IVT) reaction was incubated for 4 hr at 37°C. The yield for each transcribed sgRNA was quantified using a NanoDrop spectrophotometer. Panel B. Each sgRNA IVT reaction was a run on an Agilent Bioanalyzer to assay for sample quality.
Flow chart describing how the Guide-it sgRNA In Vitro Transcription Kit (including the Guide-it IVT RNA Clean-Up Kit) and Guide-it sgRNA Screening Kit work together in the Guide-it Complete sgRNA Screening System, which can be used to synthesize and test the efficacy of sgRNAs
Flow chart describing how the Guide-it sgRNA In Vitro Transcription Kit (including the Guide-it IVT RNA Clean-Up Kit) and Guide-it sgRNA Screening Kit work together in the Guide-it Complete sgRNA Screening System, which can be used to synthesize and test the efficacy of sgRNAs.
