TransIT-siQUEST Transfection Reagent
TransIT-siQUEST Transfection Reagent
A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells
A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells
- Broad Spectrum siRNA Delivery– Utilize one transfection reagent and protocol for a wide variety of cells
- Low Cellular Toxicity– Maintain cell density and reduce experimental biases
- Reproducible Results– Obtain consistent, targeted gene knockdowns from day to day
- High Knockdown Efficiency– Achieve optimal gene silencing in a large percentage of cells to ensure experimental success
Additional Resources
Technical Report: High Efficiency siRNA Delivery In Vitro (PDF)
Publication: Delivery of small interfering RNA to mammalian cells in culture
Poster: RNAi Applications in Nucleic Acid Delivery (PDF)
Specifications
Storage Conditions:All Configurations: Store at 4°C
Product Guarantee:
All Configurations: 1 year
Technical Product Literature
Full Protocols
TransIT-siQUEST® Full Transfection Protocol (PDF)
Quick Reference Protocols
TransIT-siQUEST® Quick Ref Protocol (PDF)
SDS
Frequently Asked Questions
Figures and Data
High Knockdown and Low Toxicity Using TransIT-siQUEST® Reagent in CHO Cells Stably Expressing Firefly Luciferase. CHO-luc cells were grown in 24 wells plates and transfected with 25 nM of either a non-targeting siRNA or a anti-firefly luciferase siRNA using the indicated reagents with the volumes noted beneath each well. Luciferase expression, normalized to non-targeting siRNA control (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as percent cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.
Delivery of Fluorescently-Labeled siRNA Using TransIT-siQUEST® Transfection Reagent. HeLa (70% confluence) cells in 12-well plates were transfected with TransIT-siQUEST® Transfection Reagent (3 μl/well) and Label IT® siRNA Tracker™ Cy-3-labeled siRNA duplexes (RED, 25 nM final concentration in the well). The cells were incubated 24 hours post-transfection then fixed and counterstained with counterstained with Alexa Fluor® 488 Phalloidin (GREEN) (Life Technologies). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope.
Efficient Target Gene Knockdown in Selected Cell Lines Using TransIT-siQUEST® Reagent. Reporter plasmids expressing both firefly and sea pansy luciferase were co-transfected into the indicated cell lines using TransIT® Plasmid Transfection Reagents. Targeted knockdown was achieved by transfection of an anti-firefly luciferase siRNA using the TransIT-siQUEST® Reagent. Twenty-four hours later, firefly luciferase expression was normalized to sea pansy luciferase expression and compared to the reagent alone contr