NucleoProtect RNA
NucleoProtect RNA
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoProtect RNA
Applications
- Convenient storage of cell and tissue samples without the need for immediate processing or shock-freezing of samples in liquid N2
- Safe, transport of stabilized cell and tissue samples without dry ice for subsequent RNA isolation
- Preservation of RNA integrity within cell and tissue samples (downstream applications: qRT-PCR, RNAseq, array technology, etc.)
Features
- Collect your sample now – get your RNA later
- Protect your cell and tissue samples from RNA degradation
- Compatible with most RNA isolation methods, incl. NucleoSpin® and NucleoMag® RNA kits as well as NucleoZOL
Specifications
- Technology: RNA stabilization reagent
- Processing: Add 10 volumes to sample or immerse sample in 10 volumes
- Sample material:
- Human / animal cells and tissues (max. 5 mm diameter)
- Storage time: ≤ 24 hours at 37 °C
- ≤ 7 days at 18–25 °C
- ≤ 1 month at 4 °C
- Long term at -20 °C or lower
- Typical RIN after RNA isolation:
- 10 for cultured mammalian cells (e.g., HeLa)
- > 9 for mammalian tissue
Collect your sample now – get your RNA later!
The NucleoProtect® RNA stabilization solution allows convenient long term storage of animal cell and tissue samples for subsequent RNA isolation. Samples are treated with NucleoProtect® RNA which permeates the tissue during incubation and protects the RNA from degradation. Samples can be stored conveniently at 4 °C for up to one month or at ≤ -20 °C for long term storage.
NucleoProtect® RNA preserves RNA integrity and is compatible with the RNA isolation method of your choice.
Procedure
Efficient stabilization of RNA in samples prior to RNA isolation
Cell culture and mouse tissue samples (fresh, stabilized, and non-stabilized) were used for subsequent RNA isolation with the NucleoSpin® RNA Plus kit. In this experimental setup NucleoProtect® RNA preserves RNA integrity within samples as good as or better than competitor solutions (MN = NucleoProtect® RNA; S = RNAlater®; Z = DNA/RNA ShieldTM).
Protection from freeze-thaw damage
Protection from freeze-thaw damage. RNA integrity in samples stabilized with NucleoProtect RNA is well-maintained even after 10 freeze-thaw cycles.
NucleoProtect RNA prevents RNA degradation during sample storage at –75 degrees C
NucleoProtect RNA prevents RNA degradation during sample storage at –75°C. Using the NucleoSpin RNA Plus kit, RNA was purified from various tissue samples that were stored at –75°C for 3 months and either unstabilized or stabilized in NucleoProtect RNA (MN) or RNAlater (S). As demonstrated, NucleoProtect RNA effectively prevents degradation of RNA that can occur in unstabilized samples stored at –75°C.
NucleoProtect RNA preserves sample integrity during storage at 37 degrees C
NucleoProtect RNA preserves sample integrity during storage at 37°C. Using the NucleoSpin RNA Plus kit, RNA was purified from tissue samples that were stored for 1 day at either 4°C or 37°C in NucleoProtect RNA. Storage in NucleoProtect RNA effectively prevented degradation of RNA even at high ambient temperatures.
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Collect your sample now – get your RNA later!
The NucleoProtect® RNA stabilization solution allows convenient long term storage of animal cell and tissue samples for subsequent RNA isolation. Samples are treated with NucleoProtect® RNA which permeates the tissue during incubation and protects the RNA from degradation. Samples can be stored conveniently at 4 °C for up to one month or at ≤ -20 °C for long term storage.
NucleoProtect® RNA preserves RNA integrity and is compatible with the RNA isolation method of your choice.
Procedure
Efficient stabilization of RNA in samples prior to RNA isolation
Cell culture and mouse tissue samples (fresh, stabilized, and non-stabilized) were used for subsequent RNA isolation with the NucleoSpin® RNA Plus kit. In this experimental setup NucleoProtect® RNA preserves RNA integrity within samples as good as or better than competitor solutions (MN = NucleoProtect® RNA; S = RNAlater®; Z = DNA/RNA ShieldTM).
Protection from freeze-thaw damage
Protection from freeze-thaw damage. RNA integrity in samples stabilized with NucleoProtect RNA is well-maintained even after 10 freeze-thaw cycles.
NucleoProtect RNA prevents RNA degradation during sample storage at –75 degrees C
NucleoProtect RNA prevents RNA degradation during sample storage at –75°C. Using the NucleoSpin RNA Plus kit, RNA was purified from various tissue samples that were stored at –75°C for 3 months and either unstabilized or stabilized in NucleoProtect RNA (MN) or RNAlater (S). As demonstrated, NucleoProtect RNA effectively prevents degradation of RNA that can occur in unstabilized samples stored at –75°C.
NucleoProtect RNA preserves sample integrity during storage at 37 degrees C
NucleoProtect RNA preserves sample integrity during storage at 37°C. Using the NucleoSpin RNA Plus kit, RNA was purified from tissue samples that were stored for 1 day at either 4°C or 37°C in NucleoProtect RNA. Storage in NucleoProtect RNA effectively prevented degradation of RNA even at high ambient temperatures.
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