Ex Taq DNA Polymerase
Ex Taq DNA Polymerase
Brand: Takara Bio.
In stock
SKU
Ex Taq DNA Polymerase
With the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR
Ex Taq DNA polymerase—a robust PCR enzyme with proofreading activity
TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.
In routine PCR applications, using Ex Taq polymerase and the Ex Taq buffer system results in higher yields of PCR products as compared to standard Taq DNA polymerase.
Several other formats of Ex Taq are available:
- An antibody-mediated hot-start version—for preventing non-specific amplification from mispriming during reaction setup.
- A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg2+), and dNTPs—for a simple PCR setup and minimal pipetting steps. A loading-dye added versions, PerfectShot Ex Taq DNA Polymerase, is also available for convenient loading of PCR products in agarose gels directly.
Overview
- DNA-proofreading activity allows for high-fidelity and high-sensitivity reactions
- Obtain a wide range of PCR products (<100 bp to 20 kb for genomic DNA targets)
- Optimized 10X Buffer and dNTPs are included—less optimization and more reproducible results than Taq
Applications
- Ideal for high-fidelity, high-yield endpoint PCR
Amplification of a 6 kb Target from E.coli Genomic DNA with either TaKaRa Taq or TaKaRa Ex Taq DNA Polymerase.
Amplification of a 6 kb Target from E.coli Genomic DNA with either TaKaRa Taq or TaKaRa Ex Taq DNA Polymerase. PCR was performed using the indicated amounts of template, with either TaKaRa Taq (Lanes T1-T4) or TaKaRa Ex Taq (Lanes E1-E4). Each 50 µL reaction contained 1.25 units of enzyme. Lanes M contains a lambda Hind III DNA Marker.
Amplification of various size fragments from a lambda DNA template with either Takara Taq or Takara Ex Taq DNA Polymerase
Amplification of various size fragments from a lambda DNA template with either Takara Taq or Takara Ex Taq DNA Polymerase. Amplification with Takara Ex Taq polymerase resulted in more product than the reactions containing Takara Taq polymerase, particularly for amplicons longer than 4 kb.
Detection of Helicobacter pylori using PCR amplification with either Takara Taq or Takara Ex Taq DNA Polymerase
Detection of Helicobacter pylori using PCR amplification with either with either Takara Taq or Takara Ex Taq DNA Polymerase. Gastric biopsy specimens were tested for the presence of H. pylori by PCR. Takara Ex Taq polymerase amplified H. pylori sequence from the specimens while Takara Taq polymerase did not. These results indicate that Takara Ex Taq polymerase minimizes the risk of false-negative results that may occur when using conventional Taq DNA polymerases.
Write Your Own Review
Amplification of a 6 kb Target from E.coli Genomic DNA with either TaKaRa Taq or TaKaRa Ex Taq DNA Polymerase.
Amplification of a 6 kb Target from E.coli Genomic DNA with either TaKaRa Taq or TaKaRa Ex Taq DNA Polymerase. PCR was performed using the indicated amounts of template, with either TaKaRa Taq (Lanes T1-T4) or TaKaRa Ex Taq (Lanes E1-E4). Each 50 µL reaction contained 1.25 units of enzyme. Lanes M contains a lambda Hind III DNA Marker.
Amplification of various size fragments from a lambda DNA template with either Takara Taq or Takara Ex Taq DNA Polymerase
Amplification of various size fragments from a lambda DNA template with either Takara Taq or Takara Ex Taq DNA Polymerase. Amplification with Takara Ex Taq polymerase resulted in more product than the reactions containing Takara Taq polymerase, particularly for amplicons longer than 4 kb.
Detection of Helicobacter pylori using PCR amplification with either Takara Taq or Takara Ex Taq DNA Polymerase
Detection of Helicobacter pylori using PCR amplification with either with either Takara Taq or Takara Ex Taq DNA Polymerase. Gastric biopsy specimens were tested for the presence of H. pylori by PCR. Takara Ex Taq polymerase amplified H. pylori sequence from the specimens while Takara Taq polymerase did not. These results indicate that Takara Ex Taq polymerase minimizes the risk of false-negative results that may occur when using conventional Taq DNA polymerases.
