A hot-start, 2X PCR master mix containing Takara Ex Taq DNA polymerase, optimized buffer, and dNTPs. Please refer to the User Manual for buffer composition. Takara Ex Taq DNA polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA.

Overview

• High-yield, high-sensitivity PCR
• Premixed PCR master mix for convenient amplification requiring minimal assembly
• Generation of PCR products having >80% cloning efficiency in T-vectors

Applications

Ideal for high-fidelity, high-yield endpoint PCR

PCR products

PCR products generated with Ex Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors.