Ex Taq DNA polymerase, hot-start version

Takara Ex Taq HS DNA Polymerase is the hot-start version of our high-performing Takara Ex Taq polymerase (a blend of Takara Taq and a proofreading exonuclease) offering high yield, excellent sensitivity, and fidelity that is 4.5-times higher than Taq polymerase. Antibody-mediated hot-start gives lower background, higher specificity, and allows room temperature reaction assembly. Takara Ex Taq HS DNA polymerase is supplied with 10X buffer (Mg2+) and dNTP mix.

Takara Ex Taq HS DNA polymerase includes a monoclonal antibody to Taq DNA Polymerase that binds to the polymerase and prevents nonspecific amplification due to mispriming and/or formation of primer dimers during reaction assembly. The antibody is then denatured during the initial PCR DNA-denaturation step, releasing the polymerase and allowing DNA synthesis to proceed. A premix Ex Taq is available for fast and sensitive real-time PCR.

Several other formats of Ex Taq are available:

• Individual components of Ex Taq enzyme mix, dNTP mixture, and 10X reaction buffer (with or without Mg2+)—for individual reaction setup and optimization.
• A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg2+), and dNTP mixture—for a simple PCR setup and minimal pipetting steps.
• A loading dye-added 2X master mix (PerfectShot Ex Taq DNA Polymerase)—for convenient loading of PCR products in agarose gels.

Overview

• Antibody-mediated hot start: lower background, increased specificity, and room-temperature reaction assembly
• High-yield and high-sensitivity PCR
• Excellent on difficult templates

Applications

• Ideal for high-fidelity, high-yield endpoint PCR
• Amplifications requiring room-temperature reaction assembly

PCR products

PCR products generated with Takara Ex Taq HS contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency in T-vectors.