Advantage GC 2 polymerase mix & PCR kit

The Advantage GC 2 Polymerase Mix is specially formulated to amplify GC-rich templates. It includes the high-yield and high-fidelity Advantage 2 enzyme blend plus a proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two additives proven to enable the amplification of templates with up to 90% GC content with minimal buffer optimization. The combination of DMSO and GC-Melt Reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques.

For a complete kit, use the Advantage GC 2 PCR Kit (Cat. # 639120, 639119) which includes the enzyme blend, GC buffers, and dNTPs, plus GC-rich control template and primers.

The Advantage GC 2 Polymerase Mix and PCR kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix.

Overview

Optimized for use with complex, GC-rich cDNA and genomic DNA templates
Generates error-free PCR products
Amplifies targets of up to 6 kb

Applications

GC-rich PCR
Long and accurate PCR
Hot-start PCR
Difficult-target PCR

Advantage GC 2 successfully amplifies GC-rich targets

Advantage GC 2 successfully amplifies GC-rich targets. A 510 bp portion of IGFR II cDNA, containing a 110 bp region with a GC content of 90%, was amplified from Human Placenta Marathon-Ready cDNA (Cat. No. 639311) using the following: Advantage 2 + 6% DMSO + 0.1 M GC-Melt (Lane 1); Advantage 2 + 1.0 M GC-Melt (Lane 2); Advantage GC 2 + 1.0 M GC-Melt (Lane 3); and Advantage 2 + 5% DMSO (Lane 4). Lane M: DNA size marker.