PrimeSTAR GXL DNA Polymerase
PrimeSTAR GXL DNA Polymerase
Brand: Takara Bio.
In stock
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PrimeSTAR GXL DNA Polymerase
High tolerence for challenging GC-rich targets, efficient amplification in the presence of excess template.
PrimeSTAR GXL DNA Polymerase
PrimeSTAR GXL DNA Polymerase provides efficient PCR amplification even for the toughest scenarios, including amplification of GC-rich targets, amplification in the presence of excess template that can contribute to nonspecific binding, and amplification of long products (up to 30 kb). The antibody-mediated hot-start feature enhances PCR specificity and sensitivity.
PrimeSTAR GXL polymerase comes with separate tubes of 5X buffer and dNTP mixture.PrimeSTAR GXL polymerase is our most robust high-fidelity polymerase available for challenging targets (GC-rich sequences, excess template, long amplicons).
Overview
- Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
- Antibody-mediated hot-start formulation
- High tolerance for GC-rich templates as well as the presence of excess nucleic acids templates
Applications
- Reliable amplification of long, GC-rich, or AT-rich sequences, such as regulatory regions and repeats
- HLA typing
- Unbiased library amplification for NGS
Termini of PCR products
- PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.
Recommended maximum amplicon length
- Up to 30 kb (human genomic DNA template)
- Up to 40 kb (lambda DNA template)
- Up to 13.5 kb (human cDNA template)
Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases
Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases. Company T’s enzyme includes buffers optimized for GC-rich templates.
PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA).
PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases
PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases.
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Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases
Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases. Company T’s enzyme includes buffers optimized for GC-rich templates.
PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA).
PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases
PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases.
