PrimeSTAR HS with GC buffer

PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content) and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.

For longer amplicons with high GC or AT content, use PrimeSTAR GXL DNA Polymerase, which is optimized to amplify up to 40 kb from certain targets.

Overview

Only 25 errors per 304 kb when using GC-rich template DNA (as determined by sequence analysis)
Higher efficiency than standard Taq polymerase
Capable of amplifying high-GC human genomic DNA targets up to 5 kb
Fast reaction time due to an increased priming efficiency
High specificity due to antibody-mediated hot-start technology

Applications

High-fidelity PCR
High-GC-content PCR
Amplification of cDNA libraries
cDNA cloning for protein expression

Application Example

Application Example. Comparison of PCR amplification efficiency and specificity between PrimeSTAR HS DNA Polymerase with GC Buffer and high-fidelity/GC-adaptive PCR enzymes from three companies. PrimeSTAR HS DNA Polymerase demonstrated superior efficiency and specificity with a GC-rich template (746 bp of the ApoE gene, with 73.9% GC content) compared to the other enzymes.