NucleoSpin RNA Clean-up
NucleoSpin RNA Clean-up
Brand: MACHEREY-NAGEL
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NucleoSpin RNA Clean-up
Applications
RNA clean-up of:
- Pre-purified RNA (e.g., Trizol®)
- Reaction mixtures
- Amino-allyl-mRNA
- Biotinylated RNA
- RNA isolation from up to 105 cultured cells (whenever co-purification of some genomic DNA is acceptable, kit does not contain rDNase)
- Typical downstream applications: enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip hybridizations
Features
- Complete removal of RT-PCR inhibitors
- Time-saving procedure based on NucleoSpin® RNA, without DNase digestions and homogenization steps
- RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions (e.g. amplification reactions, labeling reactions)
Specifications
- Technology: Silica membrane technology
- Fragment size: > 200 bp
- A260/A280: 1.9–2.1
- Sample material: Phenol / chloroform extract (< 200 μL), reaction mixture, cells (< 10⁵)
- Typical recovery: 85–95 %
- Elution volume: 40–120 μL
- Binding capacity: 200 μg
- Processing time: 20 min/6 preps
Purification of high quality RNA from reaction mixtures
RNA quality (RIN) and quantity were analyzed with an Agilent® Bioanalyzer, Agilent® RNA 6000 Nano chip, and Agilent® RNA 6000 Nano reagent before and after clean-up. The crude RNA solution (lane 1 and 2) show low RNA concentrations around 85 ng/µL. Nevertheless, the RNA is not degraded and has a high RIN of about 9.4. Lane 3 and 4 show the corresponding RNA after DNase digestion and purification with NucleoSpin® RNA Clean-up XS. RNA quality is not affected by the whole procedure showing similar RIN and high recovery of about 95 % in a total volume of 20 µL.
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Purification of high quality RNA from reaction mixtures
RNA quality (RIN) and quantity were analyzed with an Agilent® Bioanalyzer, Agilent® RNA 6000 Nano chip, and Agilent® RNA 6000 Nano reagent before and after clean-up. The crude RNA solution (lane 1 and 2) show low RNA concentrations around 85 ng/µL. Nevertheless, the RNA is not degraded and has a high RIN of about 9.4. Lane 3 and 4 show the corresponding RNA after DNase digestion and purification with NucleoSpin® RNA Clean-up XS. RNA quality is not affected by the whole procedure showing similar RIN and high recovery of about 95 % in a total volume of 20 µL.
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