Advantage RT-for-PCR Kit
Advantage RT-for-PCR Kit
Brand: Takara Bio.
In stock
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Advantage RT-for-PCR Kit
Complete kit for the synthesis of first-strand cDNA from total RNA or polyA+ RNA. The kit contains sufficient reagents for 25 first-strand cDNA synthesis reactions.
Overview
- Provides efficient first-strand cDNA synthesis from nanogram quantities of RNA in just one hour
- Allows simultaneous analysis of multiple genes from a single RNA source
- Generates more full-length cDNA; recombinant RNase inhibitor included
Applications
- Two-step RT-PCR
- Gene expression analysis
- First-strand cDNA synthesis
- Quantitative RT-PCR
- RT-PCR
The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit
The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit. The time course of lipopolysaccharide induction of iNOS mRNA in the mouse macrophage cell line RAW 264.7 was studied by isolating RNA from culture samples taken at the time points indicated (in hr). Approx. 0.4 μg was then reverse transcribed using the Advantage RT-for-PCR Kit, and 5% of the cDNA product was used as a template for PCR using the Mouse G3PDH Amplimer Set (Cat. # 639009) as a control (Panel A) and mouse iNOS primers (Panel B). 20% portions of the PCR products were run on a 1.8% EtBr/agarose gel. Lane M: ΦX174/Hae III DNA size marker.
PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors
PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors. The indicated amounts of Human Lung Total RNA (Cat. # 636507) were used for reverse transcription, and 5% of each cDNA product was amplified by PCR using the Human G3PDH Control Amplimer Set (Cat. # 639005). Lanes 1, 5 & 9: 250 ng. Lanes 2, 6 & 10: 62.5 ng. Lanes 3, 7 & 11: 16 ng. Lanes 4, 8 & 12: 4 ng. Lane M: Φ X174/Hae III DNA size marker. The results show that when 4 ng of RNA was used as the starting material, only the Advantage RT-for-PCR Kit generated a clearly visible product.
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The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit
The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit. The time course of lipopolysaccharide induction of iNOS mRNA in the mouse macrophage cell line RAW 264.7 was studied by isolating RNA from culture samples taken at the time points indicated (in hr). Approx. 0.4 μg was then reverse transcribed using the Advantage RT-for-PCR Kit, and 5% of the cDNA product was used as a template for PCR using the Mouse G3PDH Amplimer Set (Cat. # 639009) as a control (Panel A) and mouse iNOS primers (Panel B). 20% portions of the PCR products were run on a 1.8% EtBr/agarose gel. Lane M: ΦX174/Hae III DNA size marker.
PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors
PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors. The indicated amounts of Human Lung Total RNA (Cat. # 636507) were used for reverse transcription, and 5% of each cDNA product was amplified by PCR using the Human G3PDH Control Amplimer Set (Cat. # 639005). Lanes 1, 5 & 9: 250 ng. Lanes 2, 6 & 10: 62.5 ng. Lanes 3, 7 & 11: 16 ng. Lanes 4, 8 & 12: 4 ng. Lane M: Φ X174/Hae III DNA size marker. The results show that when 4 ng of RNA was used as the starting material, only the Advantage RT-for-PCR Kit generated a clearly visible product.
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