Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation. Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It gives higher PCR product yield than other polymerases, and is active over a wide range of Mg2+ concentrations.
Titanium Taq is available in several formats:
Individual components—separate tubes of Titanium Taq polymerase mix and buffer (dNTPs not included).
Glycerol-free formulation—50X Titanium Taq SP (Glycerol-Free) is ideal for preparation of custom dried-down PCR master mixes or automation workflows.
For a complete system, use the Titanium Taq PCR Kit—includes polymerase mix, PCR buffer, 50X dNTP mix, control DNA template, control primer mix, and PCR-grade water.
A ready-to-use, lyophilized 2X PCR master mix—High Yield PCR EcoDry Premix for convenient room-temperature set up, transport, and storage.
Overview
High yield—requires fewer PCR cycles, therefore generating less background signal
Active over wide ranges of Mg2+ concentrations—minimizes time spent on optimization
Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets
Integrated hot-start antibody for enhanced specificity—minimizes primer-dimer formation and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature
Lyophilized format available as EcoDry Premix—stores conveniently at room temperature. Ideal for field work or point-of-care assays
Applications
Multiplex PCR
Genotyping
Hot-start PCR
Whole-genome PCR
Rare-template amplification
Standard PCR, such as genotyping, primer extension
Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.
TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.
Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation. Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It gives higher PCR product yield than other polymerases, and is active over a wide range of Mg2+ concentrations.
Titanium Taq is available in several formats:
Individual components—separate tubes of Titanium Taq polymerase mix and buffer (dNTPs not included).
Glycerol-free formulation—50X Titanium Taq SP (Glycerol-Free) is ideal for preparation of custom dried-down PCR master mixes or automation workflows.
For a complete system, use the Titanium Taq PCR Kit—includes polymerase mix, PCR buffer, 50X dNTP mix, control DNA template, control primer mix, and PCR-grade water.
A ready-to-use, lyophilized 2X PCR master mix—High Yield PCR EcoDry Premix for convenient room-temperature set up, transport, and storage.
Overview
High yield—requires fewer PCR cycles, therefore generating less background signal
Active over wide ranges of Mg2+ concentrations—minimizes time spent on optimization
Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets
Integrated hot-start antibody for enhanced specificity—minimizes primer-dimer formation and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature
Lyophilized format available as EcoDry Premix—stores conveniently at room temperature. Ideal for field work or point-of-care assays
Applications
Multiplex PCR
Genotyping
Hot-start PCR
Whole-genome PCR
Rare-template amplification
Standard PCR, such as genotyping, primer extension
Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.
TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.