Titanium Taq PCR Kit
Titanium Taq PCR Kit
Brand: Takara Bio.
In stock
SKU
Titanium Taq PCR Kit
Titanium Taq PCR Kit
This complete kit contains all the reagents needed for PCR, including control template and primer mix. Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStar® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. Titanium Taq works with a wide range of samples, and is active over a wide range of Mg2+ concentrations.
Overview
- High yield—requires fewer PCR cycles.
- Active over wide ranges of Mg2+ concentrations—minimizes time spent on optimization.
- Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets.
- Integrated hot-start antibody for enhanced specificity—minimizes the formation of primer dimers and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature.
Applications
- Hot-start PCR
- Multiplex PCR
- Rare-template amplification
- Whole-genome PCR
- Standard PCR (e.g., genotyping, primer extension, RT-PCR, and screening clones)
Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.
TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.
References
Kellogg, D. E. et al. Taqstart Antibody: “Hot Start” PCR Facilitated by a Neutralizing Monoclonal Antibody Directed Against Taq DNA Polymerase. BioTechniques 16, 1137 (1994).
Product citations
Barnes, W. M. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29–35 (1992).
Kim, Y. et al. Crystal structure of Thermus aquaticus DNA polymerase. Nature 376, 612–616 (1995).
Write Your Own Review
Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.
TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.
