Xfect mESC Transfection Reagent
Xfect mESC Transfection Reagent
Brand: Takara Bio.
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Xfect mESC Transfection Reagent
Xfect mouse embryonic stem cell transfection reagent
Xfect mESC Transfection Reagent provides the highest transfection efficiency in multiple mES cell lines, including ES-E14TG2a and ES-D3 mES cell lines. This reagent is specifically recommended for mouse ES cells; for transfecting other cell types, including adult stem cells, we recommend Xfect Transfection Reagent.
Overview
- mES cell-specific transfection reagent (for other cell types try Xfect Transfection Reagent)
- >90% transfection efficiency
- Polymer forms biodegradable nanoparicles with plasmid DNA
- Low cytotoxicity—obtain biologically relevant data, with no undesired effects
- Simple, serum-compatible protocol
Applications
- Transfection and gene expression in mES cells.
Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent
Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent. ES-E14TG2a mES cells were transfected with 5 µg of DsRed-Express2 plasmid using 250 µg of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry (left panel) as well as phase and fluorescent microscopy (middle and right panels, respectively). As quantified by flow cytometric analysis, 93.9% of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression (left panel), with an MFI of 716.9 (data not shown).
Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells
Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells. Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encoding AcGFP1, in a 6-well format. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency.
Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents
Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents. ES-E14TG2a mES cells were transfected in a 6-well plate format with a plasmid expressing AcGFP1 using Xfect mESC Transfection Reagent (Panel A), Product L (Panel B), Product LX (Panel C), or Product F (Panel D) according to each manufacturer’s recommended protocol. 48 hr posttransfection, the cells were imaged using white light (top) and by fluorescence microscopy using a Zeiss Axioskop microscope equipped with a GFP filter (bottom).
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Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent
Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent. ES-E14TG2a mES cells were transfected with 5 µg of DsRed-Express2 plasmid using 250 µg of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry (left panel) as well as phase and fluorescent microscopy (middle and right panels, respectively). As quantified by flow cytometric analysis, 93.9% of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression (left panel), with an MFI of 716.9 (data not shown).
Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells
Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells. Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encoding AcGFP1, in a 6-well format. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency.
Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents
Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents. ES-E14TG2a mES cells were transfected in a 6-well plate format with a plasmid expressing AcGFP1 using Xfect mESC Transfection Reagent (Panel A), Product L (Panel B), Product LX (Panel C), or Product F (Panel D) according to each manufacturer’s recommended protocol. 48 hr posttransfection, the cells were imaged using white light (top) and by fluorescence microscopy using a Zeiss Axioskop microscope equipped with a GFP filter (bottom).
