Xfect Transfection Reagent
Xfect Transfection Reagent
Brand: Takara Bio.
In stock
SKU
Xfect Transfection Reagent
Xfect Transfection Reagent
An obvious way to increase transfection efficiency is to increase the amount of plasmid DNA and transfection reagent mixture that you add to your cells. Unfortunately, since most transfection reagents damage cells during transfection, simply using more of it is not an option. The Xfect reagent is different—it allows you to increase your transfection efficiency to the level you need, without killing your cells.
The Xfect reagent is a biodegradable transfection polymer, screened from a pool of 2,300 candidates for its very low cytotoxicity profile and high transfection efficiency. The protocol is far simpler than with most commercially available transfection reagents, it is performed in a single tube, in the presence of serum, and with no requirement for optimization of plasmid to polymer ratios.
The Xfect reagent also outperforms popular competitor transfection reagents in Jurkat cells, which are notoriously difficult to transfect. Xfect Transfection Reagent produced >40-fold higher transfection efficiency than Product L. This transfection reagent can also be used to transfect human adult stem cells. We used the Xfect reagent to transfect human adipose-derived stem cells (hADSCs) with a transfection efficiency of 92%.
Overview
Excellent for most cell types, even difficult-to-transfect cells
Xfect transfects the five most commonly transfected cell lines with very high efficiency:
- High transfection efficiency and very low cytotoxicity
- Transfect a broad range of cell types
- Convenient split packaging (2 x 50 rxns, or 3 x 100 rxns); share a kit with your colleagues
- Simple, single-tube, serum-compatible protocol
- No Opti-MEM needed, everything is included
- No optimization of ratios required
- 1 reaction corresponds to a transfection in a well of a 6-well plate
Applications
- Transfection reagent for most commmonly used cell lines
High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent
High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent.
Transfection of Jurkat cells
Transfection of Jurkat cells. Suspension cells such as Jurkat cells are notoriously very hard to transfect, but using the Xfect Transfection Reagent, you can achieve almost 30% efficiency. The transfection efficiency using the leading competitor product, on the other hand, is no higher than background.
Xfect transfection reagent yields higher numbers of transfected, viable cells than a popular competitor reagent
Xfect transfection reagent yields higher numbers of transfected, viable cells than a popular competitor reagent. The Xfect Reagent and Product L were each used according to their respective protocols to transfect HeLa cells with increasing amounts of plasmid DNA encoding the Living Colors fluorescent protein, AcGFP1. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency and cell viability was assessed by trypan dye exclusion assay.
Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent
Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent.
The simple Xfect Transfection Reagent protocol is completely serum-compatible
The simple Xfect Transfection Reagent protocol is completely serum-compatible.
Xfect Transfection Reagent to transfect the cell lines listed here
Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here.
Write Your Own Review
Xfect Transfection Reagent
An obvious way to increase transfection efficiency is to increase the amount of plasmid DNA and transfection reagent mixture that you add to your cells. Unfortunately, since most transfection reagents damage cells during transfection, simply using more of it is not an option. The Xfect reagent is different—it allows you to increase your transfection efficiency to the level you need, without killing your cells.
The Xfect reagent is a biodegradable transfection polymer, screened from a pool of 2,300 candidates for its very low cytotoxicity profile and high transfection efficiency. The protocol is far simpler than with most commercially available transfection reagents, it is performed in a single tube, in the presence of serum, and with no requirement for optimization of plasmid to polymer ratios.
The Xfect reagent also outperforms popular competitor transfection reagents in Jurkat cells, which are notoriously difficult to transfect. Xfect Transfection Reagent produced >40-fold higher transfection efficiency than Product L. This transfection reagent can also be used to transfect human adult stem cells. We used the Xfect reagent to transfect human adipose-derived stem cells (hADSCs) with a transfection efficiency of 92%.
Overview
Excellent for most cell types, even difficult-to-transfect cells
Xfect transfects the five most commonly transfected cell lines with very high efficiency:
- High transfection efficiency and very low cytotoxicity
- Transfect a broad range of cell types
- Convenient split packaging (2 x 50 rxns, or 3 x 100 rxns); share a kit with your colleagues
- Simple, single-tube, serum-compatible protocol
- No Opti-MEM needed, everything is included
- No optimization of ratios required
- 1 reaction corresponds to a transfection in a well of a 6-well plate
Applications
- Transfection reagent for most commmonly used cell lines
High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent
High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent.
Transfection of Jurkat cells
Transfection of Jurkat cells. Suspension cells such as Jurkat cells are notoriously very hard to transfect, but using the Xfect Transfection Reagent, you can achieve almost 30% efficiency. The transfection efficiency using the leading competitor product, on the other hand, is no higher than background.
Xfect transfection reagent yields higher numbers of transfected, viable cells than a popular competitor reagent
Xfect transfection reagent yields higher numbers of transfected, viable cells than a popular competitor reagent. The Xfect Reagent and Product L were each used according to their respective protocols to transfect HeLa cells with increasing amounts of plasmid DNA encoding the Living Colors fluorescent protein, AcGFP1. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency and cell viability was assessed by trypan dye exclusion assay.
Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent
Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent.
The simple Xfect Transfection Reagent protocol is completely serum-compatible
The simple Xfect Transfection Reagent protocol is completely serum-compatible.
Xfect Transfection Reagent to transfect the cell lines listed here
Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here.
