AAV2-Cre Recombinase
AAV2-Cre Recombinase
Brand: Takara Bio.
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AAV2-Cre Recombinase
Helper virus-free preparation of AAV particles to deliver Cre recombinase
The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) particles to deliver a Cre recombinase gene cassette to mammalian cells. This kit enables the preparation of high-titer AAV2 particles without a helper virus. Simply transfect the three included plasmids into HEK 293T cells to prepare the virus. This kit also includes an AAV Extraction Solution for efficient virus isolation.
Overview
- Helper virus-free preparation of high-titer AAV2 particles encoding the Cre recombinase gene under the control of the CMV promoter
- Enables delivery of the Cre recombinase gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
- The Cre recombinase protein mediates recombination between loxP sites, allowing gene inversion, translocation, or deletion
Applications
- Genome editing—transduction of target cells containing a floxed DNA sequence with the AAV2-CRE recombinase viral particles results in recombination between the loxP sites
- Generating transgenic mice
Analysis of ZsGreen1 expression in HEK 293 cells infected with AAV2-Cre particles
Analysis of ZsGreen1 expression in HEK 293 cells infected with AAV2-Cre particles. AAV2-Cre viral particles were prepared using the AAVpro Helper Free System (AAV2-CRE Recombinase) and purified using the AAVpro Purification Kit (AAV2). HEK293 cells engineered to express ZsGreen1 after Cre-mediated recombination were infected with either 100 vg/cell or 12,500 vg/cell of AAV2-Cre viral particles and analyzed by flow cytometry. The proportion of ZsGreen1+ cells increased with the amount of AAV2-Cre particles used for infection.
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Analysis of ZsGreen1 expression in HEK 293 cells infected with AAV2-Cre particles
Analysis of ZsGreen1 expression in HEK 293 cells infected with AAV2-Cre particles. AAV2-Cre viral particles were prepared using the AAVpro Helper Free System (AAV2-CRE Recombinase) and purified using the AAVpro Purification Kit (AAV2). HEK293 cells engineered to express ZsGreen1 after Cre-mediated recombination were infected with either 100 vg/cell or 12,500 vg/cell of AAV2-Cre viral particles and analyzed by flow cytometry. The proportion of ZsGreen1+ cells increased with the amount of AAV2-Cre particles used for infection.
