Cloning Enhancer removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning. Cloning Enhancer-treated PCR samples yield significantly more recombinant clones. Since treatment with Cloning Enhancer occurs in the same tube as the PCR reaction, it is less likely to result in UV damage or nicking. Avoiding a separate cleanup process also minimizes the risk of losing PCR products during purification.
Overview
Convenient treatment of PCR products without purification
Single-tube reaction with no sample loss
Gentle treatment—no DNA damage or nicking
5X increase in colony yield compared to unpurified inserts
Cloning Enhancer removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning. Cloning Enhancer-treated PCR samples yield significantly more recombinant clones. Since treatment with Cloning Enhancer occurs in the same tube as the PCR reaction, it is less likely to result in UV damage or nicking. Avoiding a separate cleanup process also minimizes the risk of losing PCR products during purification.
Overview
Convenient treatment of PCR products without purification
Single-tube reaction with no sample loss
Gentle treatment—no DNA damage or nicking
5X increase in colony yield compared to unpurified inserts