Overview
The In-Fusion HD Cloning Plus kit enables high-efficiency, high-fidelity directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes: a NucleoSpin Gel and PCR Clean-Up Kit, which can be used for spin-column purification of PCR products; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.
- Subcloning is unnecessary: Clone any insert, into any locus, in any vector, with just one reaction
- Highly efficient: Over 95% efficiency demonstrated with a broad range of fragment sizes, from 0.5 kb to 15 kb
- Seamless construction: Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
- Flexibility to clone single or multiple fragments: Clone single or multiple DNA fragments simultaneously, in a single reaction
- Can be used to perform site-directed mutagenesis: Click here to learn how
Applications
- PCR cloning
- High-throughput cloning (see full range of reagent options on our high-throughput cloning page)
- Multiple-fragment cloning
- Gene synthesis
- Gene design
- Mutagenesis
- Domain swapping
- Domain modification
