NucleoSpin gDNA Clean-up
NucleoSpin gDNA Clean-up
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoSpin gDNA Clean-up
Applications
- Clean-up and concentration of pre-purified DNA (organic extractions)
- Clean-up and concentration of DNA from enzymatic reactions
- Not recommended for gel or PCR clean-up! For this application, please use NucleoSpin® Gel and PCR Clean-up.
- Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification
Features
- Effective post clean-up and concentration of large DNA fragments for successful downstream applications
- High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp
- Concentrate your DNA sample easily and faster compared to microdialysis filtrations units
- 15 min/10 preps – time-saving procedure
Specifications
- Technology: Silica membrane technology
- Fragment size: 100 bp–approx. 50 kbp
- A260/A280: 1.8–1.9
- Sample material: Aqueous DNA solution (< 150 μL incl. < 25 μg DNA)
- Typical recovery: 80–90 %
- Elution volume: 50–100 μL
- Binding capacity: 50 μg
- Processing time: 10 min/6 preps
Efficient clean up of genomic DNA with minimal loss
DNA fragments with and without QIAzol® / TRIzol® contamination (1 %) were purified with the NucleoSpin® gDNA Clean-up kit (elution volume 50 μL). A spectrophotometric analysis of the input fractions (with and without QIAzol® contamination) in comparison to the purified samples demonstrates the efficient clean up with the NucleoSpin® gDNA Clean-up kit. The phenol contamination is removed entirely with minimum losses in final DNA yield, resulting in an absorbtion spectrum essentially identical to that of the non-contaminated input DNA (Input ( - QIAzol®)). Bars colored in grey / blue correspond to A230, medium grey / medium blue colored bars to A260, and light grey / light blue colored bars to A280.
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Efficient clean up of genomic DNA with minimal loss
DNA fragments with and without QIAzol® / TRIzol® contamination (1 %) were purified with the NucleoSpin® gDNA Clean-up kit (elution volume 50 μL). A spectrophotometric analysis of the input fractions (with and without QIAzol® contamination) in comparison to the purified samples demonstrates the efficient clean up with the NucleoSpin® gDNA Clean-up kit. The phenol contamination is removed entirely with minimum losses in final DNA yield, resulting in an absorbtion spectrum essentially identical to that of the non-contaminated input DNA (Input ( - QIAzol®)). Bars colored in grey / blue correspond to A230, medium grey / medium blue colored bars to A260, and light grey / light blue colored bars to A280.
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