NucleoSpin gDNA Clean-up XS
NucleoSpin gDNA Clean-up XS
Brand: MACHEREY-NAGEL
In stock
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NucleoSpin gDNA Clean-up XS
Applications
- Clean-up and concentration of pre-purified DNA (organic extractions)
- Clean-up and concentration of DNA from enzymatic reactions
- Not recommended for gel or PCR clean-up! For this application, please use NucleoSpin® Gel and PCR Clean-up.
- Typical downstream applications: PCR, enzymatic reactions, Southern blotting, qPCR, STR amplification
Features
- Efficient post clean-up of large DNA fragments for successful downstream applications
- Recommended for small DNA quantities extracted from, e.g., forensic samples
- Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA
- Easier and faster than microdialysis filtration units
- 20 min/6 preps – time-saving procedure
Specifications
- Technology: Silica membrane technology
- Fragment size: 100 bp–approx. 50 kbp
- A260/A280: 1.8–1.9
- Sample material: Aqueous DNA solution (< 400 μL incl. < 2 μg DNA)
- Typical recovery: 60–70 %
- Elution volume: 6–10 μL
- Binding capacity: 3 μg
- Processing time: 20 min/6 preps
Efficient clean up of genomic DNA with minimal loss
DNA fragments with and without QIAzol® / TRIzol® contamination (1 %) were purified with the NucleoSpin® gDNA Clean-up kit (elution volume 50 μL). A spectrophotometric analysis of the input fractions (with and without QIAzol® contamination) in comparison to the purified samples demonstrates the efficient clean up with the NucleoSpin® gDNA Clean-up kit. The phenol contamination is removed entirely with minimum losses in final DNA yield, resulting in an absorbtion spectrum essentially identical to that of the non-contaminated input DNA (Input ( - QIAzol®)). Bars colored in grey / blue correspond to A230, medium grey / medium blue colored bars to A260, and light grey / light blue colored bars to A280.
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Efficient clean up of genomic DNA with minimal loss
DNA fragments with and without QIAzol® / TRIzol® contamination (1 %) were purified with the NucleoSpin® gDNA Clean-up kit (elution volume 50 μL). A spectrophotometric analysis of the input fractions (with and without QIAzol® contamination) in comparison to the purified samples demonstrates the efficient clean up with the NucleoSpin® gDNA Clean-up kit. The phenol contamination is removed entirely with minimum losses in final DNA yield, resulting in an absorbtion spectrum essentially identical to that of the non-contaminated input DNA (Input ( - QIAzol®)). Bars colored in grey / blue correspond to A230, medium grey / medium blue colored bars to A260, and light grey / light blue colored bars to A280.
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