EmeraldAmp MAX HS PCR Master Mix

EmeraldAmp MAX HS PCR Master Mix is the antibody-mediated hot-start (HS) version of EmeraldAmp MAX PCR Master Mix. The inclusion of an antibody prevents mispriming and non-specific amplification during PCR setup.

EmeraldAmp Max HS PCR Master Mix can be used for routine PCR applications, and completed reactions can be analyzed directly via gel electrophoresis. This hot-start master mix includes a high-yield HS PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (green), and a density reagent in a 2X Taq PCR master mix format. Only primers and DNA template need to be added to initiate the reaction.

Overview

Antibody-mediated hot start—minimizes nonspecific amplification and primer dimers
High yield—at least 10 times more end product than conventional PCR
Excellent performance—optimized buffer for better performance with AT- or GC-rich targets
Convenience—simply add template, primers, and water to the 2X Taq PCR Master Mix, then load the reactions directly onto a gel upon completion of PCR
Emerald green loading dye—easy tracking during electrophoresis
Easy integration with downstream applications—restriction enzyme digests can be performed directly in the PCR buffer

Applications

TA cloning or sequencing
Direct gel electrophoresis
Long and high-yield PCR