EmeraldAmp MAX PCR Master Mix
EmeraldAmp MAX PCR Master Mix
Brand: Takara Bio.
In stock
SKU
EmeraldAmp MAX PCR Master Mix
Premixed with green loading dye for high-yield, routine and ease-of-use PCR.
EmeraldAmp MAX PCR Master Mix
The EmeraldAmp MAX PCR Master Mix offers a powerful and convenient option for high-yield, routine, and specific PCR. This premix formulation includes an optimized buffer, PCR enzyme, dNTP mixture, gel-loading dye (green), and a density reagent in a convenient 2X premix format. The buffer is optimized for better performance with AT or GC-rich targets, and allows amplification of long products. It is possible to amplify 15 kb genomic DNA fragments with this master mix.
Only primers and DNA template need to be added to initiate the reaction. Completed reactions can be analyzed directly via gel electrophoresis. The vivid green dye separates into blue and yellow dye fronts when the PCR product is run on an agarose gel.
An antibody-mediated hot-start version is available.
Overview
- Streamlined workflow—simply add template, primers, and water to the 2X master mix then load reactions directly onto gel.
- Ease-of-use—emerald green loading dye tracks migration during electrophoresis.
- High yield—obtain at least 10 times more end product.
- Long PCR—amplify targets up to 10 kb in length.
- Easy integration with downstream application—perform restriction digests directly in the PCR buffer or use in TA cloning.
Applications
- TA cloning or sequencing
- Direct gel electrophoresis
- Long and high-yield PCR
Write Your Own Review
